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Besides elucidating the construction and also the specific operate from the CupA, CupB, and CupC-affiliated adhesins, entire realization from the complexity as well as specificity on the adherence mechanisms used by P. aeruginosa
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The mutants had been analyzed by inverse PCR, utilizing two oligonucleotides, OTn1 and OTn2, to amplify adjacent DNA fragment into the transposon insertion internet site, accompanied by sequencing in the cloned solution (see Resources and Methods
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mutants defective in distinctive levels of biofilm formation, quite a few factors that take part in the initiation of biofilm formation and progression to microcolonies and mature biofilm had been recognized (nine). With the Examination with the phenotypes of various P. aeruginosa
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